Journal: The EMBO Journal

Article Title: Control of replication and gene expression by ADP-ribosylation of DNA in Mycobacterium tuberculosis

doi: 10.1038/s44318-025-00451-y

Figure Lengend Snippet: An experiment schematic is included in the panel. BCG darG-sgRNA and BCG carrying an empty vector were pretreated for 48 h with 200 ng/ml ATC, then loaded into a microfluidic device. The bacteria were imaged every hour for 59 h, with continued ATC treatment. BCG-vector control bacilli divide over 59 h ( A – C ), whereas BCG darG-sgRNA stop dividing and elongate ( D – F ). An example is circled in each treatment. .

Article Snippet: For microscopy experiments, minimally darG -silenced bacilli were harvested after 7 days of treatment and loaded into a CellAsic ONIX Microfluidic Platform using a plate for bacterial cells.

Techniques: Plasmid Preparation, Bacteria, Control

Journal: The EMBO Journal

Article Title: Control of replication and gene expression by ADP-ribosylation of DNA in Mycobacterium tuberculosis

doi: 10.1038/s44318-025-00451-y

Figure Lengend Snippet: An experiment schematic is included in the panel. BCG darG-sgRNA were minimally inhibited with ATC in a microtitre plate for 7 days, and loaded into a microfluidic device ( A ). After 12 h, ATC was washed out of the device ( B ). The bacterium marked with a white arrow was followed over time ( C ). After 16 h in fresh medium, division occurs at one pole ( D – F ). After 58 in fresh medium, branching occurs at the other pole ( G ) but is followed by swelling ( H ) and necrosis ( I ).

Article Snippet: For microscopy experiments, minimally darG -silenced bacilli were harvested after 7 days of treatment and loaded into a CellAsic ONIX Microfluidic Platform using a plate for bacterial cells.

Techniques: